Coding

Part:BBa_K3409001:Design

Designed by: Charlotte Duteil   Group: iGEM20_Ionis_Paris   (2020-10-19)


Chaperone protein gp 38


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 60
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Position 484: GAT (Asp) mutated to GAC (Asp) to remove illegal EcorI site


Source

Entire genomic sequence of the bacteriophage T4 found on NCBI GenBanK: https://www.ncbi.nlm.nih.gov/nuccore/NC_000866.4?report=genbank&to=168903

Specific sequence for gp38 found on NCBI GenBank : https://www.ncbi.nlm.nih.gov/nuccore/NC_000866.4?from=159649&to=160200

References